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Phage vB_BveM-Goe7 represents a new genus in the subfamily Bastillevirinae.

Alexandra Dominique FurrerMechthild BömekeMichael HoppertRobert Hertel
Published in: Archives of virology (2020)
Bacillus velezensis FZB42 is a Gram-positive, endospore-forming rhizobacterium that is associated with plant roots and promotes plant growth. It was used as host to isolate phage vB_BveM-Goe7 (Goe7). Goe7 exhibits a Myoviridae morphology with a contractile tail and an icosahedral head. Its genome is 158,674 bp in size and contains 5137-bp-long terminal repeats (LTRs). It also contains five tRNA-encoding genes and 251 coding DNA sequences (CDS), of which 65 were annotated. The adsorption constant of Goe7 is 6.1 ± 0.24 × 10-8 ml/min, with a latency period of 75 min and a burst size of 114 particles per burst. A BLASTn sequence comparison against the non-redundant nucleotide database of NCBI revealed that Goe7 is most similar to Bacillus subtilis phage vB_BsuM-Goe3.
Keyphrases
  • bacillus subtilis
  • pseudomonas aeruginosa
  • plant growth
  • genome wide
  • high frequency
  • quantum dots
  • single molecule
  • single cell
  • gram negative
  • optical coherence tomography
  • cell wall