Importance of protein Ser/Thr/Tyr phosphorylation for bacterial pathogenesis.
Julie Bonne KøhlerCarsten JersMériem SenissarLei ShiAbderahmane DerouicheIvan MijakovicPublished in: FEBS letters (2020)
Protein phosphorylation regulates a large variety of biological processes in all living cells. In pathogenic bacteria, the study of serine, threonine, and tyrosine (Ser/Thr/Tyr) phosphorylation has shed light on the course of infectious diseases, from adherence to host cells to pathogen virulence, replication, and persistence. Mass spectrometry (MS)-based phosphoproteomics has provided global maps of Ser/Thr/Tyr phosphosites in bacterial pathogens. Despite recent developments, a quantitative and dynamic view of phosphorylation events that occur during bacterial pathogenesis is currently lacking. Temporal, spatial, and subpopulation resolution of phosphorylation data is required to identify key regulatory nodes underlying bacterial pathogenesis. Herein, we discuss how technological improvements in sample handling, MS instrumentation, data processing, and machine learning should improve bacterial phosphoproteomic datasets and the information extracted from them. Such information is expected to significantly extend the current knowledge of Ser/Thr/Tyr phosphorylation in pathogenic bacteria and should ultimately contribute to the design of novel strategies to combat bacterial infections.
Keyphrases
- protein kinase
- mass spectrometry
- machine learning
- living cells
- multiple sclerosis
- infectious diseases
- big data
- healthcare
- escherichia coli
- staphylococcus aureus
- ms ms
- high resolution
- pseudomonas aeruginosa
- single molecule
- induced apoptosis
- liquid chromatography
- type diabetes
- metabolic syndrome
- small molecule
- health information
- radiation therapy
- lymph node
- cystic fibrosis
- candida albicans
- insulin resistance
- gas chromatography
- single cell
- tandem mass spectrometry