Characterization of humoral responses to Nipah virus infection in the Syrian Hamster model of disease.
Florine E M ScholteSergio E RodriguezStephen R WelchKatherine A DaviesSarah C GenzerJoAnn D Coleman-McCrayJessica R HarmonTeresa E SorvilloMichael K LoElif KaraaslanEric BergeronJoel M MontgomeryJessica R SpenglerChristina F SpiropoulouPublished in: The Journal of infectious diseases (2023)
Nipah virus (NiV) is a highly pathogenic paramyxovirus. The Syrian hamster model recapitulates key features of human NiV disease and is a critical tool for evaluating antivirals and vaccines. Here we describe longitudinal humoral immune responses in NiV-infected Syrian hamsters. Samples were obtained 1-28 days after infection and analyzed by ELISA, neutralization, and Fc-mediated effector function assays. NiV infection elicited robust antibody responses against the nucleoprotein and attachment glycoprotein. Levels of neutralizing antibodies were modest and only detectable in surviving animals. Fc-mediated effector functions were mostly observed in nucleoprotein-targeting antibodies. Antibody levels and activities positively correlated with challenge dose.