Flexible Amperometric Immunosensor Based on Colloidal Quantum Dots for Detecting the Myeloperoxidase (MPO) Systemic Inflammation Biomarker.
Yanbing TaoYunong ZhaoLe WangJing HuangYan ChenQing HuangBoxiang SongHua-Yao LiJianjun ChenHuan LiuPublished in: Biosensors (2023)
Myeloperoxidase (MPO) has been demonstrated to be a biomarker of neutrophilic inflammation in various diseases. Rapid detection and quantitative analysis of MPO are of great significance for human health. Herein, an MPO protein flexible amperometric immunosensor based on a colloidal quantum dot (CQD)-modified electrode was demonstrated. The remarkable surface activity of CQDs allows them to bind directly and stably to the surface of proteins and to convert antigen-antibody specific binding reactions into significant currents. The flexible amperometric immunosensor provides quantitative analysis of MPO protein with an ultra-low limit of detection (LOD) (31.6 fg mL -1 ), as well as good reproducibility and stability. The detection method is expected to be applied in clinical examination, POCT (bedside test), community physical examination, home self-examination and other practical scenarios.
Keyphrases
- label free
- loop mediated isothermal amplification
- sensitive detection
- human health
- quantum dots
- climate change
- risk assessment
- healthcare
- high resolution
- hydrogen peroxide
- mental health
- reduced graphene oxide
- real time pcr
- oxidative stress
- binding protein
- solid state
- physical activity
- protein protein
- gold nanoparticles
- nitric oxide
- mass spectrometry