Epigenetic Process Monitoring in Live Cultures with Peptide Biosensors.
Nur P DamayantiKevin BunoSherry L Voytik HarbinJoseph Maria Kumar IrudayarajPublished in: ACS sensors (2019)
Acetyltransferase is a member of the transferase group responsible for transferring an acetyl group from acetyl-CoA to amino group of a histone lysine residue. Past efforts on histone acetylation monitoring involved biochemical analysis that do not provide spatiotemporal information in a dynamic format. We propose a novel approach to monitor acetyltransferase acetylation in live single cells using time correlated single photon counting fluorescence lifetime imaging (TCSPC-FLIM) with peptide biosensors. Utilizing 2D and 3D cultures we show that the peptide sensor has a specific response to acetyltransferase enzyme activity in a fluorescence lifetime dependent manner ( P < 0.001). Our FLIM biosensor concept enables real-time longitudinal measurement of acetylation activity with high spatial and temporal resolution in live single cells to monitor cell function or evaluate drug effects to treat cancer or neurological diseases.
Keyphrases
- induced apoptosis
- dna methylation
- cell cycle arrest
- single molecule
- gene expression
- endoplasmic reticulum stress
- gold nanoparticles
- high resolution
- healthcare
- papillary thyroid
- oxidative stress
- cell death
- emergency department
- young adults
- energy transfer
- amino acid
- quality improvement
- squamous cell carcinoma
- squamous cell
- cell proliferation
- social media
- health information