Xenopus pitx3 target genes lhx1 and xnr5 are identified using a novel three-fluor flow cytometry-based analysis of promoter activation and repression.
Lara N HookerCristine SmoczerSamuel AbbottMohamad FakhereddinJohn W HudsonMichael J CrawfordPublished in: Developmental dynamics : an official publication of the American Association of Anatomists (2017)
We demonstrate how flow cytometry can be used to detect gene regulatory changes with exquisite precision on a cell-by-cell basis, and establish that in HEK293 cells, pitx3 directly activates lhx1 and represses xnr5. Developmental Dynamics 246:657-669, 2017. © 2017 Wiley Periodicals, Inc.