Hydrogels with Cell Adhesion Peptide-Decorated Channel Walls for Cell Guidance.
Shuang WangMariah SarwatPeng WangDenver C SurraoDamien G HarkinJames A St JohnEleonore C L BolleAurelien ForgetPaul D DaltonTim R DargavillePublished in: Macromolecular rapid communications (2020)
A method is reported for making hollow channels within hydrogels decorated with cell-adhesion peptides exclusively at the channel surface. Sacrificial fibers of different diameters are used to introduce channels within poly(ethylene glycol) hydrogels crosslinked with maleimide-thiol chemistry, which are backfilled with a cysteine-containing peptide solution which is conjugated to the lumen with good spatial efficiency. This allows for peptide patterning in only the areas of the hydrogel where they are needed when used as cell-guides, reducing the amount of required peptide 20-fold when compared to bulk functionalization. The power of this approach is highlighted by successfully using these patterned hydrogels without active perfusion to guide fibroblasts and olfactory ensheathing cells-the latter having unique potential in neural repair therapies.
Keyphrases
- cell adhesion
- hyaluronic acid
- drug delivery
- extracellular matrix
- tissue engineering
- wound healing
- drug release
- single cell
- cell therapy
- induced apoptosis
- magnetic resonance imaging
- quantum dots
- computed tomography
- signaling pathway
- cell cycle arrest
- mass spectrometry
- climate change
- oxidative stress
- high resolution
- endoplasmic reticulum stress
- human health
- cell death
- fluorescent probe
- pi k akt
- contrast enhanced