Three neo-clerodane diterpenoids, including two new tinocordifoliols A (1) and B (2) and one known tinopanoid R (3), were isolated from the ethyl acetate-soluble fraction of the 70% ethanol extract of Tinospora cordifolia stems. The structures were elucidated by various spectroscopic methods, including one dimensional (1D) and 2D-NMR, high resolution-electrospray ionization (HR-ESI)-MS, and electronic circular dichroism (ECD) data. The T. cordifolia extract and all isolated compounds 1-3 possessed arginase I inhibitory activities. Among them, 3 exhibited moderate competitive inhibition of human arginase I (IC 50 = 61.9 µM). Furthermore, docking studies revealed that the presence of a β-substituted furan in 3 may play a key role in the arginase I inhibitory activities.
Keyphrases
- high resolution
- nitric oxide synthase
- ms ms
- molecular docking
- mass spectrometry
- oxidative stress
- endothelial cells
- nitric oxide
- magnetic resonance
- molecular dynamics
- anti inflammatory
- multiple sclerosis
- molecular dynamics simulations
- single cell
- electronic health record
- big data
- tandem mass spectrometry
- ionic liquid
- solid state