In vitro and in vivo studies of Syzygium cumini -loaded electrospun PLGA/PMMA/collagen nanofibers for accelerating topical wound healing.
Esraa B AbdelazimTasneem AbedShaimaa S GoherShaza H AlyHeba A S El-NasharShahira Hussainy El-MoslamyEsmail M El-FakharanyEnas A Abdul-BakiMarwa Mosaad ShakweerNoura G EissaMahmoud ElsabahyElbadawy A KamounPublished in: RSC advances (2024)
This work aims to develop plant extract-loaded electrospun nanofiber as an effective wound dressing scaffolds for topical wound healing. Electrospun nanofibers were fabricated from Syzygium cumini leaf extract (SCLE), poly(lactic- co -glycolic acid) (PLGA), poly(methyl methacrylate) (PMMA), collagen and glycine. Electrospinning conditions were optimized to allow the formation of nanosized and uniform fibers that display smooth surface. Morphology and swelling behavior of the formed nanofibers were studied. In addition, the antibacterial activity of the nanofibers against multidrug-resistant and human pathogens was assessed by agar-well diffusion. Results showed that nanofibers containing Syzygium cumini extract at concentrations of 0.5 and 1% w/v exhibited greater antibacterial activity against the tested Gram-positive ( i.e. , Staphylococcus aureus , Candida albicans , Candida glabrata and Bacillus cereus ) and Gram-negative ( i.e. , Salmonella paratyphi and Escherichia coli ) pathogens compared to the same concentrations of the plain extract. Furthermore, in vivo wound healing was evaluated in Wistar rats over a period of 14 days. In vivo results demonstrated that nanofiber mats containing SCLE and collagen significantly improved wound healing within two weeks, compared to the control untreated group. These findings highlight the potential of fabricated nanofibers in accelerating wound healing and management of topical acute wounds.
Keyphrases
- wound healing
- gram negative
- multidrug resistant
- candida albicans
- biofilm formation
- escherichia coli
- drug resistant
- staphylococcus aureus
- acinetobacter baumannii
- oxidative stress
- klebsiella pneumoniae
- drug delivery
- endothelial cells
- pseudomonas aeruginosa
- drug release
- liver failure
- intensive care unit
- climate change
- acute respiratory distress syndrome
- bone regeneration
- silver nanoparticles
- aortic dissection
- cystic fibrosis
- case control
- bacillus subtilis
- antimicrobial resistance