A genetically encoded near-infrared fluorescent calcium ion indicator.

Yong QianKiryl D PiatkevichBenedict Edward Mc LarneyAhmed S AbdelfattahSohum Mehta Mitchell H MurdockSven GottschalkRosana S MolinaWei ZhangYingche ChenJiahui WuMikhail DrobizhevThomas E Hughes Jin ZhangEric R SchreiterShy ShohamDaniel RazanskyEdward S BoydenRobert E Campbell

Published in: Nature methods (2019)

We report an intensiometric, near-infrared fluorescent, genetically encoded calcium ion (Ca2+) indicator (GECI) with excitation and emission maxima at 678 and 704 nm, respectively. This GECI, designated NIR-GECO1, enables imaging of Ca2+ transients in cultured mammalian cells and brain tissue with sensitivity comparable to that of currently available visible-wavelength GECIs. We demonstrate that NIR-GECO1 opens up new vistas for multicolor Ca2+ imaging in combination with other optogenetic indicators and actuators.

Keyphrases

photodynamic therapy
high resolution
fluorescence imaging
quantum dots
living cells
fluorescent probe
protein kinase
drug release
label free
flow cytometry
mass spectrometry
resting state
single molecule
cerebral ischemia