The fabrication of multifunctional sodium alginate scaffold incorporating ibuprofen-loaded modified PLLA microspheres based on cryogenic 3D printing.

A strategy to develop a multifunctional sodium alginate personalized scaffold with enhanced mechanical stability, osteogenesis activity and excellent anti-inflammatory activity by cryogenic 3 D printing combined with subsequent crosslinking with Sr 2+ is proposed in this study. The ink for 3 D printing was prepared by dispersing modified PLLA droplets containing ibuprofen into sodium alginate aqueous solution using lecithin as stabilizer. The results showed that the drug-loaded microspheres formed from the low-temperature solidifying of the modified PLLA droplets were homogeneously dispersed in sodium alginate substrate, and the scaffold displayed a sustained drug release performance toward ibuprofen which endowed the scaffold with persistent anti-inflammatory effects. In vitro cell culture indicated that the lecithin not only acted as the stabilizer, but also stimulated the proliferation and mineralization of osteoblastic cells on the scaffold. Sr 2+ -crosslinking improved the mechanical properties and osteogenic activity of the scaffold.