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Patterns of recombination in snakes reveal a tug-of-war between PRDM9 and promoter-like features.

Carla HogeMarc de ManuelMohamed MahgoubNaima OkamiWilliam J BeldenShreya M BanerjeeZachary BakerMorgan McNultyPeter AndolfattoTodd S MacfarlanMolly SchumerAthanasia C TzikaMolly Przeworski
Published in: Science (New York, N.Y.) (2024)
In some mammals, notably humans, recombination occurs almost exclusively where the protein PRDM9 binds, whereas in vertebrates lacking an intact PRDM9 , such as birds and canids, recombination rates are elevated near promoter-like features. To determine whether PRDM9 directs recombination in nonmammalian vertebrates, we focused on an exemplar species with a single, intact PRDM9 ortholog, the corn snake ( Pantherophis guttatus ). Analyzing historical recombination rates along the genome and crossovers in pedigrees, we found evidence that PRDM9 specifies the location of recombination events, but we also detected a separable effect of promoter-like features. These findings reveal that the uses of PRDM9 and promoter-like features need not be mutually exclusive and instead reflect a tug-of-war that is more even in some species than others.
Keyphrases
  • dna repair
  • dna damage
  • dna methylation
  • transcription factor
  • gene expression
  • genome wide
  • single cell