microRNA profiling in lung tissue and bronchoalveolar lavage of cigarette smoke-exposed mice and in COPD patients: a translational approach.
Griet ConickxFrancisco Avila CobosMaarten van den BergeAlen FaizHidde J HaismaPieter S HiemstraGuy F JoosGuy G O BrussellePieter MestdaghKen R BrackePublished in: Scientific reports (2017)
Chronic obstructive pulmonary disease (COPD) is characterized by a progressive airflow limitation and is associated with a chronic inflammatory response in both airways and lungs. microRNAs (miRNAs) are often highly conserved between species and have an intricate role within homeostatic conditions and immune responses. Also, miRNAs are dysregulated in smoking-associated diseases. We investigated the miRNA profile of 523 miRNAs by stem-loop RT-qPCR in lung tissue and cell-free bronchoalveolar lavage (BAL) supernatant of mice exposed to air or cigarette smoke (CS) for 4 or 24 weeks. After 24 weeks of CS exposure, 31 miRNAs were differentially expressed in lung tissue and 78 in BAL supernatant. Next, we correlated the miRNA profiling data to inflammation in BAL and lung, obtained by flow cytometry or ELISA. In addition, we surveyed for overlap with newly assessed miRNA profiles in bronchial biopsies and with previously assessed miRNA profiles in lung tissue and induced sputum supernatant of smokers with COPD. Several miRNAs showed concordant differential expression between both species including miR-31*, miR-155, miR-218 and let-7c. Thus, investigating miRNA profiling data in different compartments and both species provided accumulating insights in miRNAs that may be relevant in CS-induced inflammation and the pathogenesis of COPD.
Keyphrases
- chronic obstructive pulmonary disease
- cell free
- lung function
- cell proliferation
- inflammatory response
- long non coding rna
- oxidative stress
- immune response
- flow cytometry
- cystic fibrosis
- long noncoding rna
- single cell
- diabetic rats
- newly diagnosed
- mycobacterium tuberculosis
- ejection fraction
- transcription factor
- electronic health record
- multiple sclerosis
- machine learning
- metabolic syndrome
- toll like receptor
- gestational age
- lipopolysaccharide induced
- air pollution
- prognostic factors
- endothelial cells
- dendritic cells
- high resolution
- mass spectrometry
- lps induced
- adipose tissue
- ultrasound guided
- patient reported