Progesterone and β-hCG Determination Using an Electrochemical Combo-Strip for Pregnancy Monitoring.
Serena LaschiPatrick Severin SfraganoFrancesco RanaldiIlaria PalchettiPublished in: International journal of molecular sciences (2023)
The development of analytical devices that can allow an easy, rapid and cost-effective measurement of multiple markers, such as progesterone and β-hCG, could have a role in decreasing the burden associated with pregnancy-related complications, such as ectopic pregnancies. Indeed, ectopic pregnancies are a significant contributor to maternal morbidity and mortality in both high-income and low-income countries. In this work, an effective and highly performing electrochemical strip for a combo determination of progesterone and β-hCG was developed. Two immunosensing approaches were optimized for the determination of these two hormones on the same strip. The immunosensors were realized using cost-effective disposable electrode arrays and reagent-saving procedures. Each working electrode of the array was modified with both the IgG anti-β-hCG and anti-progesterone, respectively. By adding the specific reagents, progesterone or β-hCG can then be determined. Fast quantitative detection was achieved, with the analysis duration being around 1 h. Sensitivity and selectivity were assessed with a limit of detection of 1.5 × 10 -2 ng/mL and 2.45 IU/L for progesterone and β-hCG, respectively. The proposed electrochemical combo-strip offers great promise for rapid, simple, cost-effective, and on-site analysis of these hormones and, thus, for the development of a point-of-care diagnostic tool for early detection of pregnancy-related complications.
Keyphrases
- weight loss
- molecularly imprinted
- pregnancy outcomes
- preterm birth
- estrogen receptor
- loop mediated isothermal amplification
- label free
- gold nanoparticles
- solid phase extraction
- ionic liquid
- risk factors
- high resolution
- gestational age
- physical activity
- mental health
- real time pcr
- mass spectrometry
- high density
- single cell