Recent developments in Phos-tag electrophoresis for the analysis of phosphoproteins in proteomics.
Hisashi HiranoJun ShirakawaPublished in: Expert review of proteomics (2022)
BIS, N,N'-methylenebis(acrylamide); CBB, Coomassie brilliant blue R250; ESI, electrospray ionization; hnRNP, heterogeneous ribonucleoprotein K; LTQ-Orbitrap, Linear trap quadrupole-Orbitrap; LC, liquid chromatography; MS, mass spectrometry; MALDI, matrix-assisted laser desorption ionization; Phos-tag, phosphate-binding tag [1,3-bis [bis (pyridine-2-ylmethyl) amino] propane-2-olate]; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; TOF, time of flight; 2D-DIGE, fluorescence-labeled two-dimensional difference gel electrophoresis; 2-DE, two-dimensional gel electrophoresis.
Keyphrases
- mass spectrometry
- liquid chromatography
- high resolution mass spectrometry
- tandem mass spectrometry
- gas chromatography
- high performance liquid chromatography
- simultaneous determination
- ionic liquid
- ultra high performance liquid chromatography
- high resolution
- capillary electrophoresis
- ms ms
- wound healing
- solid phase extraction
- hyaluronic acid
- pet imaging
- dna binding
- multiple sclerosis
- energy transfer
- pet ct