A Rapid, Shallow Whole Genome Sequencing Workflow Applicable to Limiting Amounts of Cell-Free DNA.

Rebecca C AllsoppKaren PageBana AmbasagerMarc K WadsleyEmmanuel AcheampongTumisang P NterekeQi GuoGurdeep Matharu LallKelly L T GleasonEvie WrenGeorgios NteliopoulosAmelia J RushtonR Charles CoombesJacqueline A Shaw

Published in: Clinical chemistry (2023)

The ReproSeq PGS assay is rapid, at approximately $120 per sample, providing both a sCNA profile and estimation of the tumor DNA fraction from limiting cfDNA template (300pg) and individual CTCs. The approach could be used to examine the copy number landscape over time to guide treatment decisions, support future trial designs, and be applied to low volume blood spot samples enabling remote monitoring.

Keyphrases

copy number
mitochondrial dna
genome wide
loop mediated isothermal amplification
circulating tumor cells
circulating tumor
high throughput
clinical trial
study protocol
cell free
single molecule
single cell
phase ii
molecularly imprinted
smoking cessation