Discovery of immunosuppressive Lupane-type Triterpenoids from Hypericum longistylum .

The phytochemistry study of Hypericum longistylum afforded one new degraded lupane-type triterpenoid, triterhyper A ( 1 ), and seven known congeners ( 2 - 8 ). The structures of those natural products were identified by extensive spectroscopic techniques and single crystal diffraction tests. Notably, triterhyper A ( 1 ) possessing an unusual 28- nor -lupane skeleton. More significantly, compounds 1 - 3 exhibited potential inhibitory effects on murine splenocytes proliferation stimulated by anti-CD3/anti-CD28 monoclonal antibodies (mAbs) and lipopolysaccharide (LPS), with IC 50 values ranging from (4.56 ± 0.45) μM to (18.34 ± 2.34) μM, highlighting that those isolates as potential lead compounds in the development of immunosuppressive drugs for autoimmune disease treatment.