Intracellular flow cytometry staining of antibody-secreting cells using phycoerythrin-conjugated antibodies: pitfalls and solutions.

Patrick RennerMichael CroneMatthew KornasKimAnh T PioliPeter D Pioli

Published in: Antibody therapeutics (2022)

Increasing the number of centrifugation steps performed on ASCs post-fixation leads to inappropriate retention of phycoerythrin-containing antibody conjugates during ICFC.

Keyphrases

flow cytometry
induced apoptosis
cell cycle arrest
minimally invasive
photodynamic therapy
endoplasmic reticulum stress
cell death
oxidative stress
cancer therapy
signaling pathway
drug delivery