The Effects of Preeclamptic Milieu on Cord Blood Derived Endothelial Colony-Forming Cells.
Eva HallLaura AlderferErin NeuSanjoy SahaEllie JohandesDavid M HaasLaura S HanelineDonny Hanjaya-PutraPublished in: bioRxiv : the preprint server for biology (2023)
Preeclampsia is one of the leading causes of infant and maternal mortality worldwide. Many infants born from preeclamptic pregnancies are born prematurely with higher risk of developing cardiovascular later in their life. A key mechanism by which these complications occur is through stress-induced dysfunction of endothelial progenitor cells (EPCs), including endothelial colony-forming cells (ECFCs). To gain insight into this, cord blood derived ECFCs isolated from preeclamptic pregnancies (PRECs) were analyzed and compared to their healthy counterparts. While PRECs preserve key endothelial markers, they upregulate several markers associated with oxidative stress and inflammatory response. Compared to ECFCs, PRECs also exhibit lower migratory behaviors and impaired angiogenic potential. Interestingly, treatment of neuropilin-1 can improve tube formation in vitro . Collectively, this study reports that preeclamptic milieu influence phenotypes and functionality of PRECs, which can be rejuvenated using exogenous molecules. Promising results from this study warrant future investigations on the prospect of the rejuvenated PRECs to improve lung function of infants born from preeclamptic pregnancies.
Keyphrases
- cord blood
- gestational age
- induced apoptosis
- oxidative stress
- lung function
- stress induced
- birth weight
- endothelial cells
- inflammatory response
- preterm birth
- pregnancy outcomes
- low birth weight
- cell cycle arrest
- chronic obstructive pulmonary disease
- cystic fibrosis
- risk factors
- air pollution
- pregnant women
- current status
- signaling pathway
- type diabetes
- endoplasmic reticulum stress
- cardiovascular disease
- pi k akt
- physical activity
- adverse drug
- cell proliferation
- cell death
- diabetic rats
- heat shock