Blood taken immediately after fatal resuscitation attempts yields higher quality DNA for genetic studies as compared to autopsy samples.

Caroline StanasiukAndreas BrodehlSören HommJan PerssonLars HoltzAxel WittmerHenrik FoxThorsten LaserRalph KnöllGreta Marie PohlLech PaluszkiewiczThomas JakobBernd Bachmann-MennengaDietrich HenzlerSteffen GrautoffGunter VeitKarin KlingelErika HoriUdo KellnerBernd KargerStefanie SchlepperHeidi PfeifferJan GummertAnna GärtnerJens Tiesmeier

Published in: International journal of legal medicine (2023)

Isolation yield and DNA integrity from blood samples collected by the EMS is superior in comparison to autopsy specimens. DNA from blood samples collected by the EMS on scene is stable at room temperature or even for days at 4 °C. We conclude that the EMS personnel should always save a blood sample of young fatal OHCA cases died on scene to enable subsequent genetic analysis.

Keyphrases

room temperature
circulating tumor
cell free

single molecule
cardiac arrest

gene expression
emergency medical

genome wide
middle aged