Portable And Rapid Dual-Biomarker Detection Using Solution-Gated Graphene Field Transistors in The Accurate Diagnosis of Prostate Cancer.

Prostate-specific antigen (PSA) is the common serum-relevant biomarker for early prostate cancer (PCa) detection in clinical diagnosis. However, it is difficult to accurately diagnose PCa in the early stage due to the low specificity of PSA. Herein, we designed a new solution-gated graphene field transistor (SGGT) biosensor with dual-gate for dual-biomarker detection. The sensing mechanism is that the designed aptamers immobilized on the surface of the gate electrodes can capture PSA and sarcosine (SAR) biomolecules and induce the capacitance changes of the electric double layers (EDLs) of SGGT. The limit of detections (LODs) of PSA and SAR biomarkers can reach 0.01 fg/mL which is 3-4 orders of magnitude lower than previously reported assays. The detection time of PSA and SAR is ≈ 4.5 and ≈13 minutes which is significantly faster than the detection time (1-2 h) of conventional methods. The clinical serum samples testing demonstrates that the biosensor can distinguish the PCa patients from the control group and the diagnosis accuracy can reach 100%. The SGGT biosensor can be integrated into the portable platform and the diagnostic results can directly display on the smartphone/Pad. Therefore, the integrated portable platform of the biosensor can distinguish cancer types through the dual-biomarker detection. This article is protected by copyright. All rights reserved.