Catalytically Active Hollow Fiber Membranes with Enzyme-Embedded Metal-Organic Framework Coating.
Daniel Josef BellMonika WieseAriel Augusto SchönbergerMatthias WesslingPublished in: Angewandte Chemie (International ed. in English) (2020)
Metal-organic frameworks (MOFs) are suitable enzyme immobilization matrices. Reported here is the in situ biomineralization of glucose oxidase (GOD) into MOF crystals (ZIF-8) by interfacial crystallization. This method is effective for the selective coating of porous polyethersulfone microfiltration hollow fibers on the shell side in a straightforward one-step process. MOF layers with a thickness of 8 μm were synthesized, and fluorescence microscopy and a colorimetric protein assay revealed the successful inclusion of GOD into the ZIF-8 layer with an enzyme concentration of 29±3 μg cm-2 . Enzymatic activity tests revealed that 50 % of the enzyme activity is preserved. Continuous enzymatic reactions, by the permeation of β-d-glucose through the GOD@ZIF-8 membranes, showed a 50 % increased activity compared to batch experiments, emphasizing the importance of the convective transport of educts and products to and from the enzymatic active centers.
Keyphrases
- metal organic framework
- hydrogen peroxide
- single molecule
- high throughput
- optical coherence tomography
- single cell
- blood glucose
- gold nanoparticles
- nitric oxide
- high resolution
- ionic liquid
- type diabetes
- amino acid
- high speed
- molecular dynamics simulations
- sensitive detection
- binding protein
- protein protein
- living cells
- room temperature
- skeletal muscle
- small molecule
- insulin resistance
- simultaneous determination
- aqueous solution
- oxide nanoparticles