Unified platform for genetic and serological detection of COVID-19 with single-molecule technology.
Noa FurthShay ShiloNiv CohenNir ErezVadim FedyukAlexander M SchragerAdina WeinbergerAmiel A DrorAsaf ZigronMona ShehadehEyal SelaSamer SroujiSharon AmitItzchak LevyEran SegalRony DahanDan JonesDaniel C DouekEfrat ShemaPublished in: PloS one (2021)
The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a proof-of-concept for a single-molecule based, enzyme-free assay for detection of SARS-CoV-2. The unified platform we developed allows direct detection of the viral genetic material from patients' samples, as well as their immune response consisting of IgG and IgM antibodies. Thus, it establishes a platform for diagnostics of COVID-19, which could also be adjusted to diagnose additional pathogens.
Keyphrases
- single molecule
- sars cov
- high throughput
- atomic force microscopy
- living cells
- coronavirus disease
- loop mediated isothermal amplification
- immune response
- end stage renal disease
- respiratory syndrome coronavirus
- high resolution
- real time pcr
- label free
- chronic kidney disease
- ejection fraction
- newly diagnosed
- prognostic factors
- peritoneal dialysis
- dna methylation
- antimicrobial resistance
- gene expression
- patient reported
- fluorescence imaging