Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS) peak intensity normalization for complex mixture analyses.
Allison M ThompsonKelly G StrattonLisa M BramerNicole S ZavoshyLee Ann McCuePublished in: Rapid communications in mass spectrometry : RCM (2021)
Although there is no single, best normalization method for all data sets, SPANS provides a mechanism to identify an appropriate normalization method for analyzing FT-ICR-MS data quantitatively. The number of peaks used in the background distributions of SPANS contributes more significantly to the reproducibility of results than the p-value thresholds used to obtain those peaks.