Luciferases with Tunable Emission Wavelengths.

Julien HiblotQiuliyang YuMarina D B SabbadiniLuc ReymondLin XueAlberto SchenaOlivier SallinNicholas HillRudolf GrissKai Johnsson

Published in: Angewandte Chemie (International ed. in English) (2017)

We introduce luciferases whose emission maxima can be tuned to different wavelengths by chemical labeling. The luciferases are chimeras of NanoLuc with either SNAP-tag or HaloTag7. Labeling of the self-labeling tag with a fluorophore shifts the emission maximum of NanoLuc to that of the fluorophore. Luciferases with tunable colors have applications as reporter genes, for the construction of biosensors and in bioimaging.

Keyphrases

fluorescent probe
living cells
solid state
genome wide
quantum dots
energy transfer
gene expression
light emitting