AFM and FluidFM Technologies: Recent Applications in Molecular and Cellular Biology.
Mohamed-Yassine AmarouchJaouad El HilalyDriss MazouziPublished in: Scanning (2018)
Atomic force microscopy (AFM) is a widely used imaging technique in material sciences. After becoming a standard surface-imaging tool, AFM has been proven to be useful in addressing several biological issues such as the characterization of cell organelles, quantification of DNA-protein interactions, cell adhesion forces, and electromechanical properties of living cells. AFM technique has undergone many successful improvements since its invention, including fluidic force microscopy (FluidFM), which combines conventional AFM with microchanneled cantilevers for local liquid dispensing. This technology permitted to overcome challenges linked to single-cell analyses. Indeed, FluidFM allows isolation and injection of single cells, force-controlled patch clamping of beating cardiac cells, serial weighting of micro-objects, and single-cell extraction for molecular analyses. This work aims to review the recent studies of AFM implementation in molecular and cellular biology.
Keyphrases
- atomic force microscopy
- single molecule
- living cells
- single cell
- high speed
- induced apoptosis
- rna seq
- high resolution
- cell cycle arrest
- cell adhesion
- primary care
- high throughput
- endoplasmic reticulum stress
- healthcare
- stem cells
- fluorescent probe
- heart failure
- signaling pathway
- cell therapy
- bone marrow
- optical coherence tomography
- mass spectrometry
- circulating tumor cells
- case control