Flexible Multiplane Structured Illumination Microscope with a Four-Camera Detector.
Karl A JohnsonDaniel NobleRosa MachadoTristan C PaulGuy M HagenPublished in: Photonics (2022)
Fluorescence microscopy provides an unparalleled tool for imaging biological samples. However, producing high-quality volumetric images quickly and without excessive complexity remains a challenge. Here, we demonstrate a four-camera structured illumination microscope (SIM) capable of simultaneously imaging multiple focal planes, allowing for the capture of 3D fluorescent images without any axial movement of the sample. This setup allows for the acquisition of many different 3D imaging modes, including 3D time lapses, high-axial-resolution 3D images, and large 3D mosaics. We imaged mitochondrial motions in live cells, neuronal structure in Drosophila larvae, and imaged up to 130 μm deep into mouse brain tissue. After SIM processing, the resolution measured using one of the four cameras improved from 357 nm to 253 nm when using a 30×/1.05 NA objective.
Keyphrases
- high resolution
- convolutional neural network
- single molecule
- deep learning
- optical coherence tomography
- high speed
- induced apoptosis
- photodynamic therapy
- oxidative stress
- quantum dots
- weight gain
- mass spectrometry
- fluorescence imaging
- cell cycle arrest
- body mass index
- computed tomography
- blood brain barrier
- endoplasmic reticulum stress
- pi k akt
- solid state