Rescue of Glycosylphosphatidylinositol-Anchored Protein Biosynthesis Using Synthetic Glycosylphosphatidylinositol Oligosaccharides.
Paula A GuerreroYoshiko MurakamiAnkita MalikPeter H SeebergerTaroh KinoshitaDaniel Varón SilvaPublished in: ACS chemical biology (2021)
The attachment of proteins to the cell membrane using a glycosylphosphatidylinositol (GPI) anchor is a ubiquitous process in eukaryotic cells. Deficiencies in the biosynthesis of GPIs and the concomitant production of GPI-anchored proteins lead to a series of rare and complicated disorders associated with inherited GPI deficiencies (IGDs) in humans. Currently, there is no treatment for patients suffering from IGDs. Here, we report the design, synthesis, and use of GPI fragments to rescue the biosynthesis of GPI-anchored proteins (GPI-APs) caused by mutation in genes involved in the assembly of GPI-glycolipids in cells. We demonstrated that the synthetic fragments GlcNAc-PI (1), Man-GlcN-PI (5), and GlcN-PI with two (3) and three lipid chains (4) rescue the deletion of the GPI biosynthesis in cells devoid of the PIGA, PIGL, and PIGW genes in vitro. The compounds allowed for concentration-dependent recovery of GPI biosynthesis and were highly active on the cytoplasmic face of the endoplasmic reticulum membrane. These synthetic molecules are leads for the development of treatments for IGDs and tools to study GPI-AP biosynthesis.
Keyphrases
- induced apoptosis
- cell cycle arrest
- cell wall
- end stage renal disease
- endoplasmic reticulum
- newly diagnosed
- endoplasmic reticulum stress
- oxidative stress
- chronic kidney disease
- peritoneal dialysis
- prognostic factors
- small molecule
- genome wide
- cell death
- transcription factor
- binding protein
- protein protein
- replacement therapy