Multiplex Immunofluorescence in Formalin-Fixed Paraffin-Embedded Tumor Tissue to Identify Single-Cell-Level PI3K Pathway Activation.

Konrad H StopsackYing HuangSvitlana TyekuchevaTravis A GerkeClyde BangoHabiba ElfandyMichaela BowdenKathryn L PenneyThomas M RobertsGiovanni ParmigianiPhilip W KantoffLorelei A MucciMassimo Loda

Published in: Clinical cancer research : an official journal of the American Association for Cancer Research (2020)

Our novel pathway-focused approach to quantifying single-cell-level immunofluorescence in FFPE tissue identifies prostate tumors with PI3K pathway activation that are more aggressive and may respond to pathway inhibitors.

Keyphrases

single cell
prostate cancer
high throughput
rna seq
genome wide
dna methylation
gene expression
real time pcr