In Vivo Assessment of Decellularized Porcine Myocardial Slice as an Acellular Cardiac Patch.
Mickey ShahPawan KcGe ZhangPublished in: ACS applied materials & interfaces (2019)
Acellular cardiac patches made of various biomaterials have shown to improve heart function after myocardial infarction (MI). Extracellular matrix scaffold derived from a decellularized tissue has unique advantages to serve as an acellular cardiac patch due to its biomimetic nature. In this study, we examined the therapeutic outcomes of using a decellularized porcine myocardium slice (dPMS) as an acellular patch in a rat acute MI model. dPMSs with two different thicknesses (300 and 600 μm) were patched to the infarcted area of the rat myocardium, and their effects on cardiac function and host interactions were assessed. We found that the implanted dPMS firmly attached to host myocardium after implantation and prevented thinning of the left ventricular (LV) wall after an MI. A large number of host cells were identified to infiltrate into the implanted dPMS, and a significant number of vessel structures was observed in the dPMS and infarcted area. We detected a significantly higher density of M2 macrophages in the groups treated with dPMSs as compared to the MI group. Contraction of the LV wall and cardiac functional parameters (left ventricular ejection fraction and fractional shortening) was significantly improved in the treatment groups (300 and 600 μm dPMS) 4 weeks after surgery. Our results proved the therapeutic benefits of using dPMS as an acellular cardiac patch for the treatment of acute myocardial infarction.
Keyphrases
- left ventricular
- extracellular matrix
- acute myocardial infarction
- aortic stenosis
- heart failure
- ejection fraction
- hypertrophic cardiomyopathy
- cardiac resynchronization therapy
- mitral valve
- tissue engineering
- left atrial
- oxidative stress
- breast reconstruction
- induced apoptosis
- skeletal muscle
- cell cycle arrest
- liver failure
- endoplasmic reticulum stress
- type diabetes
- cell death
- percutaneous coronary intervention
- acute respiratory distress syndrome
- smooth muscle
- adipose tissue
- pi k akt
- mechanical ventilation