Coumarin-based fluorescent probe for the rapid detection of peroxynitrite 'AND' biological thiols.

A coumarin-based novel 'AND' logic fluorescent probe ROS-AHC has been developed for the simultaneous detection of ONOO - and biological thiols. ROS-AHC was shown to exhibit only a very small fluorescence response upon addition of a single GSH or ONOO - analyte. Exposure to both analytes, however, resulted in a significant fluorescence enhancement.