mRNA Turnover Protein 4 Is Vital for Fungal Pathogenicity and Response to Oxidative Stress in Sclerotinia sclerotiorum .
Chenghuizi YangLan TangLei QinWeiping ZhongXianyu TangXin GongWenqi XieYifu LiShitou XiaPublished in: Pathogens (Basel, Switzerland) (2023)
Ribosome assembly factors have been extensively studied in yeast, and their abnormalities may affect the assembly process of ribosomes and cause severe damage to cells. However, it is not clear whether mRNA turnover protein 4 (MRT4) functions in the fungal growth and pathogenicity in Sclerotinia sclerotiorum . Here, we identified the nucleus-located gene SsMRT4 using reverse genetics, and found that knockdown of SsMRT4 resulted in retard mycelia growth and complete loss of pathogenicity. Furthermore, mrt4 knockdown mutants showed almost no appressorium formation and oxalic acid production comparing to the wild-type and complementary strains. In addition, the abilities to ROS elimination and resistance to oxidative and osmotic stresses were also seriously compromised in mrt4 mutants. Overall, our study clarified the role of Ss MRT4 in S. sclerotiorum , providing new insights into ribosome assembly in regulating pathogenicity and resistance to environmental stresses of fungi.
Keyphrases
- wild type
- oxidative stress
- biofilm formation
- induced apoptosis
- binding protein
- dna damage
- bone mineral density
- escherichia coli
- cell wall
- protein protein
- cell death
- cell cycle arrest
- multidrug resistant
- endoplasmic reticulum stress
- ischemia reperfusion injury
- copy number
- genome wide
- signaling pathway
- candida albicans
- dna methylation
- transcription factor
- postmenopausal women
- genome wide identification
- quality control
- pi k akt