Altered coordination in a blue copper protein upon association with redox partner revealed by carbon-deuterium vibrational probes.

Claire C MammoserRyan E AghNicholas M GarciaYiqi WangMegan C Thielges

Published in: Physical chemistry chemical physics : PCCP (2022)

Proteins tune the reactivity of metal sites; less understood is the impact of association with a redox partner. We demonstrate the utility of carbon-deuterium labels for selective analysis of delicate metal sites. Introduced into plastocyanin, they reveal substantial strengthening of the key Cu-Cys89 bond upon association with cytochrome f .

Keyphrases

small molecule
single cell
dna methylation
density functional theory
single molecule
photodynamic therapy
human immunodeficiency virus
hiv testing
protein protein
hiv infected
aqueous solution
transition metal