Solid-State NMR of PEGylated Proteins.
Enrico RaveraSilvia CiambellottiLinda CerofoliniTommaso MartelliTatiana KozyrevaCaterina BernacchioniStefano GiuntiniMarco FragaiPaola TuranoClaudio LuchinatPublished in: Angewandte Chemie (International ed. in English) (2016)
PEGylated proteins are widely used in biomedicine but, in spite of their importance, no atomic-level information is available since they are generally resistant to structural characterization approaches. PEGylated proteins are shown here to yield highly resolved solid-state NMR spectra, which allows assessment of the structural integrity of proteins when PEGylated for therapeutic or diagnostic use.