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Molecular architecture and dynamics of ASH1 mRNA recognition by its mRNA-transport complex.

Franziska Theresia EdelmannAndreas SchlundtRoland Gerhard HeymAndreas JennerAnnika Niedner-BoblenzMuhammad Ibrahim SyedJean-Christophe PaillartRalf StehleRobert JanowskiMichael SattlerRalf-Peter JansenDierk Niessing
Published in: Nature structural & molecular biology (2017)
mRNA localization is an essential mechanism of gene regulation and is required for processes such as stem-cell division, embryogenesis and neuronal plasticity. It is not known which features in the cis-acting mRNA localization elements (LEs) are specifically recognized by motor-containing transport complexes. To the best of our knowledge, no high-resolution structure is available for any LE in complex with its cognate protein complex. Using X-ray crystallography and complementary techniques, we carried out a detailed assessment of an LE of the ASH1 mRNA from yeast, its complex with its shuttling RNA-binding protein She2p, and its highly specific, cytoplasmic complex with She3p. Although the RNA alone formed a flexible stem loop, She2p binding induced marked conformational changes. However, only joining by the unstructured She3p resulted in specific RNA recognition. The notable RNA rearrangements and joint action of a globular and an unfolded RNA-binding protein offer unprecedented insights into the step-wise maturation of an mRNA-transport complex.
Keyphrases
  • binding protein
  • high resolution
  • stem cells
  • healthcare
  • computed tomography
  • transcription factor
  • single molecule
  • molecular dynamics
  • dna damage
  • blood brain barrier
  • cell therapy
  • heat shock protein
  • dna binding