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DNA barcoding of shrimps from a mangrove biodiversity hotspot.

Jamsari Amirul Firdaus JamaluddinNoor Adelyna Mohammed AkibSiti Zuliana AhmadSiti Amalia Aisyah Abdul HalimNoor Khalidah Abdul HamidSiti Azizah Mohd Nor
Published in: Mitochondrial DNA. Part A, DNA mapping, sequencing, and analysis (2019)
A total of 74 shrimp specimens were sequenced at a 584 bp segment of the cytochrome oxidase subunit I (COI) gene to examine patterns of DNA barcode variation in a mangrove biodiversity hotspot. The Maximum Likelihood tree, barcode gap analysis, Automatic Barcode Gap Discovery analysis and sequence comparisons with data available from Barcode of Life Data System and GenBank recovered 18 taxa of which 15 were identified to species level, 2 at genus level and a single taxon at order level. Two deep mitochondrial DNA lineage divergences were found in the giant tiger prawn, Penaeus monodon. It is suggested that one of the lineages is a consequence of an introduction from aquaculture activity. These results have provided a reliable barcode library for cataloguing shrimps in this area.
Keyphrases
  • mitochondrial dna
  • copy number
  • circulating tumor
  • electronic health record
  • single molecule
  • small molecule
  • deep learning
  • machine learning
  • high throughput
  • data analysis