Cell-penetrating poly(disulfide)-based nanoquenchers ( q CPDs) for self-monitoring of intracellular gene delivery.

Wenjie LangSi Si LiewShuyi WangDawei HongLiquan ZhuShubo DuLinye JiangShao Q Yao Jingyan Ge

Published in: Chemical communications (Cambridge, England) (2022)

Monitoring gene delivery has significant benefits in gene therapy. Herein, we report a nanoquencher system by doping a FRET pair during nucleic acid-assisted cell penetrating poly(disulfide) (CPD) formation. Our results show that this strategy not only produces an efficient gene delivery polymer with minimal endolysosomal trapping, but also enables monitoring the release of the gene from the vehicle in live cells. This study further expanded the application of CPDs as promising tools in gene delivery.

Keyphrases

gene therapy
nucleic acid
single cell
induced apoptosis
cell therapy
cell cycle arrest
oxidative stress
gene expression
copy number
signaling pathway
cell proliferation
mesenchymal stem cells
cell death
reactive oxygen species
transcription factor
quantum dots