Cancer-Specific Biomarker hNQO1-Activatable Fluorescent Probe for Imaging Cancer Cells In Vitro and In Vivo.
Surendra Reddy PunganuruHanumantha Rao MadalaViswanath ArutlaKalkunte S SrivenugopalPublished in: Cancers (2018)
Human NAD(P)H quinone oxidoreductase-1 (hNQO1) is an important cancer-related biomarker, which shows significant overexpression in malignant cells. Developing an effective method for detecting NQO1 activity with high sensitivity and selectivity in tumors holds a great potential for cancer diagnosis, treatment, and management. In the present study, we report a new dicyanoisophorone (DCP) based fluorescent probe (NQ-DCP) capable of monitoring hNQO1 activity in vitro and in vivo in both ratiometric and turn-on model. NQ-DCP was prepared by conjugating dicyanoisophorone fluoroprobe with hNQO1 activatable quinone propionic acid (QPA), which remain non-fluorescent until activation by tumor-specific hNQO1. NQ-DCP featured a large Stokes shift (145 nm), excellent biocompatibility, cell permeability, and selectivity towards hNQO1 allowed to differentiate cancer cells from healthy cells. We have successfully employed NQ-DCP to monitor non-invasive endogenous hNQO1 activity in brain tumor cells in vitro and in xenografted tumors developed in nude mice.
Keyphrases
- fluorescent probe
- living cells
- papillary thyroid
- induced apoptosis
- squamous cell
- endothelial cells
- cell cycle arrest
- type diabetes
- stem cells
- lymph node metastasis
- cell proliferation
- high resolution
- white matter
- mesenchymal stem cells
- cell death
- blood brain barrier
- quantum dots
- mass spectrometry
- functional connectivity
- childhood cancer
- climate change
- skeletal muscle
- cell therapy
- signaling pathway
- resting state
- high fat diet induced
- young adults
- smoking cessation