The role of N6-methyladenosine modification on diapause in silkworm (Bombyx mori) strains that exhibit different voltinism.
Tao JiangJiashuang LiPing QianPeng XueJin XuYanrong ChenJuan ZhuShunming TangQiaoling ZhaoHeying QianXingjia ShenPublished in: Molecular reproduction and development (2019)
N6-methyladenosine (m6 A) plays a key role in regulating gene expression in myriad organisms. Diapause is an important plastic phenotype that allows insects to survive under specific environmental conditions. However, the diapause molecular mechanism remains unknown. In this study, we analyzed the phylogenetics of genes related to the m6 A modification complex in the silkworm (Bombyx mori) based on identified sequences from other organisms. We detected the expression of these genes during different developmental phases from four strains with different voltinism. We also determined total m6 A content in cells treated with different diapause hormone concentrations or eggs exposed to hydrochloric acid. Our data revealed that m6 A-modification-related gene expression and m6 A content were greater in diapause-destinated compared to nondiapause-destined strains. Our findings suggest that m6 A modification may provide significant epigenetic regulation of diapause-related genes in the silkworm.
Keyphrases
- gene expression
- escherichia coli
- dna methylation
- genome wide
- induced apoptosis
- gram negative
- risk assessment
- single cell
- bioinformatics analysis
- multidrug resistant
- genome wide identification
- deep learning
- binding protein
- endoplasmic reticulum stress
- artificial intelligence
- human health
- genome wide analysis
- pi k akt