Clinical validation of an in-house quantitative real time PCR assay for cytomegalovirus infection using the 1st WHO International Standard in kidney transplant patients.

Cássia Ferreira Braz CaurioOdelta S AllendeRoger KistKênya L SantosIzadora C S VasconcellosFranciéli P RozalesDaiane F Dalla LanaBruno M PraetzelAna Paula AlegrettiAlessandro Cumaru Pasqualotto

Published in: Jornal brasileiro de nefrologia : 'orgao oficial de Sociedades Brasileira e Latino-Americana de Nefrologia (2021)

CMV Pp65 antigenemia and CMV qPCR showed fair agreement and a moderate correlation in this study. The in-house qPCR was revealed to be an accurate method to determine CMV DNAemia in kidney transplant patients, resulting in positive results weeks before antigenemia.

Keyphrases

end stage renal disease
newly diagnosed
chronic kidney disease
prognostic factors
high resolution
high throughput
patient reported outcomes
single cell
mass spectrometry
patient reported