CTC-Race: Single-Cell Motility Assay of Circulating Tumor Cells from Metastatic Lung Cancer Patients.
Yang LiuWujun ZhaoJamie HodgsonMary EganChristen N Cooper PopeGlenda HicksPetros G NikolinakosLeidong MaoPublished in: ACS nano (2024)
Distinctive subpopulations of circulating tumor cells (CTCs) with increased motility are considered to possess enhanced tumor-initiating potential and contribute to metastasis. Single-cell analysis of the migratory CTCs may increase our understanding of the metastatic process, yet most studies are limited by technical challenges associated with the isolation and characterization of these cells due to their extreme scarcity and heterogeneity. We report a microfluidic method based on CTCs' chemotactic motility, termed as CTC-Race assay, that can analyze migrating CTCs from metastatic non-small-cell lung cancer (NSCLC) patients with advanced tumor stages and enable concurrent biophysical and biochemical characterization of them with single-cell resolution. Analyses of motile CTCs in the CTC-Race assay, in synergy with other single cell characterization techniques, could provide insights into cancer metastasis.
Keyphrases
- circulating tumor cells
- single cell
- high throughput
- circulating tumor
- rna seq
- small cell lung cancer
- squamous cell carcinoma
- biofilm formation
- induced apoptosis
- papillary thyroid
- pseudomonas aeruginosa
- cell cycle arrest
- climate change
- radiation therapy
- single molecule
- cell proliferation
- squamous cell
- signaling pathway
- epidermal growth factor receptor