Jamming Transitions in Astrocytes and Glioblastoma Are Induced by Cell Density and Tension.
Urszula HohmannJulian Cardinal von WiddernChalid GhadbanMaria Cristina Lo GiudiceGrégoire LemahieuElisabetta Ada Cavalcanti AdamFaramarz DehghaniTim HohmannPublished in: Cells (2022)
Collective behavior of cells emerges from coordination of cell-cell-interactions and is important to wound healing, embryonic and tumor development. Depending on cell density and cell-cell interactions, a transition from a migratory, fluid-like unjammed state to a more static and solid-like jammed state or vice versa can occur. Here, we analyze collective migration dynamics of astrocytes and glioblastoma cells using live cell imaging. Furthermore, atomic force microscopy, traction force microscopy and spheroid generation assays were used to study cell adhesion, traction and mechanics. Perturbations of traction and adhesion were induced via ROCK or myosin II inhibition. Whereas astrocytes resided within a non-migratory, jammed state, glioblastoma were migratory and unjammed. Furthermore, we demonstrated that a switch from an unjammed to a jammed state was induced upon alteration of the equilibrium between cell-cell-adhesion and tension from adhesion to tension dominated, via inhibition of ROCK or myosin II. Such behavior has implications for understanding the infiltration of the brain by glioblastoma cells and may help to identify new strategies to develop anti-migratory drugs and strategies for glioblastoma-treatment.
Keyphrases
- single cell
- cell therapy
- cell adhesion
- induced apoptosis
- atomic force microscopy
- cell cycle arrest
- single molecule
- high throughput
- photodynamic therapy
- staphylococcus aureus
- multiple sclerosis
- mass spectrometry
- drug induced
- cell proliferation
- binding protein
- cell death
- blood brain barrier
- candida albicans
- optical coherence tomography
- cerebral ischemia