Discrepancy of glutamic acid decarboxylase 65 autoantibody results between RSR radioimmunoassay and enzyme-linked immunosorbent assay in patients with type 1 diabetes is related to autoantibody affinity.

Eiji KawasakiAkira OkadaAira UchidaTakahiro FukuyamaYoko SagaraYuko NakanoHidekazu TamaiMasayuki TojikuboNobuhiko Koga

Published in: Journal of diabetes investigation (2019)

The RSR-RIA test identifies both high- and low-affinity GADA, whereas the RSR-ELISA test identifies only high-affinity GADA. A longer initial incubation time in the RSR-ELISA test increases the sensitivity of GADA with the same specificity in patients with type 1 diabetes.

Keyphrases

genome wide
high throughput
gene expression
monoclonal antibody
mass spectrometry