Two Leishmania species separation targeting the ITS-rDNA and Cyt b genes by developing and evaluating HRM- qPCR.
Elnaz AlaeenovinParviz ParviziSeyedeh Maryam GhafariPublished in: Revista da Sociedade Brasileira de Medicina Tropical (2022)
Precise identification of Leishmania parasites is crucial for strategies of disease control. Real-time PCR using EvaGreen provides rapid, highly sensitive, and specific detection of parasite's DNA. The modified High-Resolution Melting could determine unique curves and was able to detect single nucleotide polymorphisms according to small differences in the nucleotide content of Leishmania parasites.
Keyphrases
- real time pcr
- high resolution
- plasmodium falciparum
- loop mediated isothermal amplification
- bioinformatics analysis
- mass spectrometry
- genome wide
- circulating tumor
- single molecule
- liquid chromatography
- cancer therapy
- cell free
- drug delivery
- high speed
- fluorescent probe
- tandem mass spectrometry
- molecularly imprinted
- trypanosoma cruzi