A Molecular Test for Quantifying Functional Notch Signaling Pathway Activity in Human Cancer.
Kirsten Canté-BarrettLaurent HoltzerHenk van OoijenRico HagelaarValentina CordoʹWim F J VerhaeghAnja van de StolpeJules P P MeijerinkPublished in: Cancers (2020)
High Notch pathway activity was not limited to T-ALL samples harboring strong NOTCH1 mutations, including juxtamembrane domain mutations or hetero-dimerization combined with PEST-domain or FBXW7 mutations, indicating that additional mechanisms may activate Notch signaling. The measured Notch pathway activity was related to intracellular NOTCH levels, indicating that the pathway activity score more accurately reflects Notch pathway activity than when it is predicted on the basis of NOTCH1 mutations. Importantly, patients with low Notch pathway activity had a significantly shorter event-free survival compared to patients showing higher activity.