Cholesterol regulates plasma membrane bending by prominin-family proteins.

-glycosylation states. A distant homolog of Prom1 called Ttyh1 is also shown to form extracellular vesicles and supports more membrane bending than Prom1 though it does not bind cholesterol. This work unites the Prom and Ttyh protein families into a single functional group and helps explain how membrane cholesterol functionalizes prominin-family proteins for different roles in different cell types.