Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01.
Maria Cristina Pinheiro Pereira Reis-MansurJanine S Cardoso-RurrJosemar V Maiworm Abreu SilvaGabriela Rodrigues de SouzaVerônica da Silva CardosoFelipe Raposo Passos MansoldoYuri PinheiroJúnia SchultzLuciene B Lopez BalottinAntonio Jorge Ribeiro da SilvaClaudia LageElisabete Pereira Dos SantosAlexandre Soares RosadoAlane Beatriz VermelhoPublished in: Scientific reports (2019)
The Microbacterium sp. LEMMJ01 isolated from Antarctic soil does not belong to any of the nearest species identified in the RDP database. Under UV radiation (A, B and C wavebands) the survival fractions of Microbacterium sp. cells were much higher compared with wild-type E. coli K12A15. Especially remarkable for an Antarctic bacterium, an expressive resistance against high UV-B doses was observed. The increased survival of DNA repair-proficient E. coli grown overnight added of 0.1 mg/ml or 1 mg/ml of the whole pigment extract produced by Microbacterium sp. revealed that part of the resistance of Microbacterium sp. against UV-B radiation seems to be connected with photoprotection by its pigments. Scanning electron microscopy revealed that UV-A and UV-B ensued membrane alterations only in E. coli. The APCI-MS fingerprints revealed the diagnostic ions for neurosporene (m/z 580, 566, 522, 538, and 524) synergism for the first time in this bacterium by HPLC-MS/MS analysis. Carotenoids also were devoid of phototoxicity and cytotoxicity effects in mouse cells and in human keratinocytes and fibroblasts.
Keyphrases
- ms ms
- dna repair
- induced apoptosis
- electron microscopy
- aqueous solution
- escherichia coli
- single cell
- mass spectrometry
- endothelial cells
- oxidative stress
- multiple sclerosis
- high resolution
- radiation therapy
- endoplasmic reticulum stress
- emergency department
- adverse drug
- free survival
- liquid chromatography tandem mass spectrometry
- radiation induced
- induced pluripotent stem cells
- simultaneous determination
- drug induced