DNA methylation profiling of genomic DNA isolated from urine in diabetic chronic kidney disease: A pilot study.

Ashani R LecamwasamAlexandra Sexton-OatesJake CarmodyElif I EkinciKaren M DwyerRichard Saffery

Published in: PloS one (2018)

High quality data can be obtained provided a sufficient yield of genomic DNA is isolated. Despite optimizing various extraction methodologies, the modest amount of genomic DNA derived from urine, may limit the generalisability of this approach for the identification of DNA methylation biomarkers of chronic diabetic kidney disease.

Keyphrases

dna methylation
circulating tumor
copy number
cell free
chronic kidney disease
single molecule
genome wide
type diabetes
gene expression
nucleic acid
wound healing
end stage renal disease
circulating tumor cells
single cell
deep learning
peritoneal dialysis