Amyloid-like Behavior of Site-Specifically Citrullinated Myelin Oligodendrocyte Protein (MOG) Peptide Fragments inside EBV-Infected B-Cells Influences Their Cytotoxicity and Autoimmunogenicity.
Can AramanMiriam E van GentNico J MeeuwenoordNicole HeijmansMikkel H S MarqvorsenWard DoelmanBart W FaberBert A 't HartSander Izaäk van KasterenPublished in: Biochemistry (2019)
Multiple sclerosis (MS) is an autoimmune disorder manifested via chronic inflammation, demyelination, and neurodegeneration inside the central nervous system. The progressive phase of MS is characterized by neurodegeneration, but unlike classical neurodegenerative diseases, amyloid-like aggregation of self-proteins has not been documented. There is evidence that citrullination protects an immunodominant peptide of human myelin oligodendrocyte glycoprotein (MOG34-56) against destructive processing in Epstein-Barr virus-infected B-lymphocytes (EBV-BLCs) in marmosets and causes exacerbation of ongoing MS-like encephalopathies in mice. Here we collected evidence that citrullination of MOG can also lead to amyloid-like behavior shifting the disease pathogenesis toward neurodegeneration. We observed that an immunodominant MOG peptide, MOG35-55, displays amyloid-like behavior upon site-specific citrullination at positions 41, 46, and/or 52. These amyloid aggregates are shown to be toxic to the EBV-BLCs and to dendritic cells at concentrations favored for antigen presentation, suggesting a role of amyloid-like aggregation in the pathogenesis of progressive MS.
Keyphrases
- multiple sclerosis
- epstein barr virus
- white matter
- diffuse large b cell lymphoma
- mass spectrometry
- dendritic cells
- ms ms
- oxidative stress
- endothelial cells
- type diabetes
- chronic obstructive pulmonary disease
- immune response
- metabolic syndrome
- adipose tissue
- case report
- insulin resistance
- skeletal muscle
- small molecule