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Shape selective bifacial recognition of double helical DNA.

Shivaji A ThadkeV M HridyaJ Dinithi R PereraRoberto R GilArnab MukherjeeDanith H Ly
Published in: Communications chemistry (2018)
An impressive array of antigene approaches has been developed for recognition of double helical DNA over the past three decades; however, few have exploited the 'Watson-Crick' base-pairing rules for establishing sequence-specific recognition. One approach employs peptide nucleic acid as a molecular reagent and strand invasion as a binding mode. However, even with integration of the latest conformationally-preorganized backbone design, such an approach is generally confined to sub-physiological conditions due to the lack of binding energy. Here we report the use of a class of shape-selective, bifacial nucleic acid recognition elements, namely Janus bases, for targeting double helical DNA or RNA. Binding occurs in a highly sequence-specific manner under physiologically relevant conditions. The work may provide a foundation for the design of oligonucleotides for targeting the secondary and tertiary structures of nucleic acid biopolymers.
Keyphrases
  • nucleic acid
  • cancer therapy
  • single molecule
  • circulating tumor
  • cell free
  • high throughput
  • cell migration
  • transcription factor
  • high density